Health & Environmental Research Online (HERO)


Print Feedback Export to File
1619911 
Journal Article 
Measurement of enzyme kinetics on the intact skin--new method to study the biological effects of cosmetics on the epidermis 
Pugliese, PT 
1978 
0 
Journal of the Society of Cosmetic Chemists
ISSN: 0037-9832 
IPA/79/322207 
Soc 
REF 16 
177-184 
eng 
IPA COPYRIGHT: ASHP A new direct fluorometric method is described which allows the measurement of enzyme activity on the intact skin of various body surfaces. This permits normal physiological parameters to operate on the system studied. The pentose phosphate pathway (Entner-Doudoroff), previously shown to operate in the epidermis, provides several enzymes which are used here to assess and compare various dermatological conditions. Glucose-6-phosphate dehydrogenase and lactic dehydrogenase are measured by fluorometric determination of changes in NADPH and NADH. Other enzymes of the Embden Myerhoff and Krebs cycles are measured directly or indirectly by this method. Various cosmetic base ingredients and compounded formulations were studied to determine their effects on epidermal metabolism. Enzyme action was recorded as increased, decreased or not affected. This new method is simple and relatively inexpensive, and allows extremely wide applications. Cosmetic agents studied included mineral oil, lanolin alcohol, isopropyl palmitate, and sodium lauryl ether sulfate (sodium laureth sulfate). 
Fluorometry; enzymes; skin; Skin; fluorometry; Kinetics; skin; Cosmetics; effects; enzymes; Mineral oil; enzymes; Lanolin alcohol; Isopropyl palmitate; Sodium laureth sulfate; Methodology; effects; Sodium lauryl ether sulfate