Health & Environmental Research Online (HERO)


Print Feedback Export to File
1620409 
Technical Report 
Reactivation of isopropyl-methylphosphonylated acetylcholinesterase by alpha,omega-bis-(4-hydroxyiminomethylpyridinium)-2-trans-butene dibromide. The effect of temperature 
Patocka, J 
1972 
IMPERIAL CHEM INDUS LTD 
HAPAB/73/01398 
Pharmacol 
23 
HAPAB Acetylcholinesterase prepared from bovine erythrocytes was inhibited by incubation for 30 min at 25 DEG with isopropylmethylphosphonofluoridate (sarin). The reactivation was performed with alpha,omega-bis-(4-hydroxyiminomethylpyridinium)2-trans-butene dibromide at a concentration of 0.01 to 0.1 mM and pH 8.0 in a sodium phosphate buffer, over a temperature range between 5 and 40 DEG C. Bimolecular reaction rate constants, free energies, and entropies of reactivation were determined at the temperatures investigated. The activation energy was calculated as kcal/mole, based on the temperature dependence of the reaction rates, and is similar to values reported in the literature for diisonitrosoacetone, pralidoxime methiodide, and isonitrosoacetophenone.