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HERO ID
1624758
Reference Type
Journal Article
Title
Simultaneous high performance liquid chromatography determination of three sulfonylureas in plasma
Author(s)
Al-Hazaymeh, NI; Lubbad, AA
Year
2001
Is Peer Reviewed?
Yes
Journal
Journal of the College of Physicians and Surgeons - Pakistan
ISSN:
1022-386X
Volume
11
Issue
5 (May 2001)
Page Numbers
304-307
Abstract
A high-performance liquid chromatographic (HPLC) method has been developed for the simultaneous analysis of three frequently used sulfonylureas (chlorpropamide, glipizide and glibenclamide) by using a reversed phase C-8 column with a mobile phase consisting of 0.1% Ortho-phosphoric acid pH 2.7: isopropanol: acetonitrile, (45:25:30) operated at ambient temperature, with analysis time less than ten minutes. The eluted drugs were monitored by UV at 235nm. The extraction was performed by using dichloromethane after the plasma sample was mixed with the buffer (0.1% orthophosphoric acid).The overall recoveries and relative standard deviations were (93.7 plus or minus 5.3)% for chlorpropamide, (91.5 plus or minus 4.9)% for glipizide and (95 plus or minus 2.8)% for glibenclamide. The response was linear in the range (0.1-100mg/ml) for chlorpropamide, glipizide, and glibenclamide, with r_ > 0.999 for all drugs. Detection limits were 2ng/ml plasma for chlorpropamide, 15ng/ml plasma for glipizide and 7ng/ml plasma for glibenclamide, measured at a Signal/Noise (S/N) of 3. No interference from administered drugs (barbiturates, b-blockers, Tranquillizer, Antihypertensive, Histamine antagonist, Antidepressant, anti emetic, and anticonvulsant) or endogenous constituents were observed.
Keywords
glipizide; sulfonylurea; glyburide; chlorpropamide; High-performance liquid chromatography
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