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1627836 
Journal Article 
The Direct Enantiomeric Determination Of (-)And (+)-Propranolol In Human Serum By High-Performance Liquid Chromatography On A Chiral Stationary Phase 
Wainer, IW; Doyle, TD; Donn, KH; Powell, JR 
1984 
1 
Journal of Chromatography
ISSN: 0021-9673 
NIOSH/00143416 
306 
405-411 
A method was developed for determining enantiomeric purity of propanolol (525666) using a chiral stationary phase with high performance liquid chromatography (HPLC). Racemic and enantiomerically pure 3-isopropyl-5-naphthoxy-2-oxazolidones were synthesized. Phosgene was added and the organic layer was collected, dried, and evaporated. A human subject was dosed with 80 milligrams of racemic propanolol-hydrochloride (318989) and blood samples were collected at intervals from 0.5 to 12 hours after administration. Chromatograms were taken of whole blood extracts before and after dosing. Assays were performed in whole blood samples spiked with 50 nanograms per milliliter (ng/ml) racemic propanolol. Standard curves were prepared and efficiency and reproducibility were investigated. Extraction efficiency was greater than 99 percent. The reaction of propanolol and phosgene produced a rigid oxazolidone ring system. The cyclization proceeded without racemization and the enantiomers were directly resolved using HPLC with a chiral stationary phase. The authors conclude that the analytical approach is a direct and rapid probe of the enantiomeric purity of propanolol. The approach can be used with samples ranging from nanograms to milligrams in size and is applicable to biological samples. 
DCN-130848; Mass spectrometry; Trace analysis; Laboratory techniques; Chromatographic analysis; Analytical methods; Quantitative analysis; Pharmacology; Aryls; Nitrogen compounds; Research; Analytical chemistry