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1630184 
Journal Article 
Reversed-phase liquid chromatography of egg white proteins. Optimization of ovalbumin elution 
Nau, F; Mallard, A; Pages, J; Brule, G 
1999 
Yes 
Journal of Liquid Chromatography and Related Technologies
ISSN: 1082-6076
EISSN: 1520-572X 
22 
8 
1129-1147 
WOS:000080174800003 
This paper is an experimental contribution to the
optimization of ovalbumin elution in RP-HPLC with a C4 support. The effects of TFA concentration,
gradient duration, flow rate, and the nature of the solvent were studied using an experimental
design. The results highlighted the major role of TFA concentration in the recovery yield of
ovalbumin, with an optimum concentration range which is very narrow and much lower (0.025%) than
the levels usually applied (0.1%). In this respect, ovalbumin seems to behave in a specific way.
Among the four solvents tested, i.e. acetonitrile, isopropanol, ethanol, and a mixture of these
three, the first one is definitely the most satisfactory in the area of recovery yield and
efficiency. By modifying the elution conditions alone, the recovery yield of ovalbumin may range
from 50 to 85%. Finally, this work has enabled us to define the chromatographic conditions for
quantitative egg white analysis of its major proteins in only six minutes and with minimal
subsequent ghosting effects.