US EPA

1630680 

Journal Article 

HPLC fluorescence determination of nitrites in water using precolumn derivatization with 4-methyl-7-aminocoumarin 

Zhou, JY; Prognon, P; Dauphin, C; Hamon, M 

1993 

Yes 

Chromatographia
ISSN: 0009-5893
EISSN: 1612-1112 

FRIEDR VIEWEG SOHN VERLAG GMBH 

WIESBADEN 1 

36 (1993) 

1 

57-60 

English 

10.1007/BF02263837 

WOS:A1993KX19400010 

A rapid and sensitive method is described for the determination of nitrites in water. Nitrite was derivatized with 4-methyl-7-aminocoumarin in sulphuric acid medium to give the corresponding 7-diazo compound which was, in turn, hydrolysed to the highly fluorescent 4-methyl-7-hydroxycoumarin. The latter compound was detected fluorimetrically at 380 nm after excitation at 325 nm. High-performance liquid chromatography (HPLC) was required to separate 4-methyl-7-hydroxycoumarin from excess 4-methyl-7-aminocoumarin. Separation was optimized on a cyano stationary phase with apolar hexane-isopropanol (95 to 5 volume per volume) as eluent. Response curves were linear in the nitrite concentration range 1 to 30 ug per litre and the limit of detection was 0.5 ug per litre. The respective repeatability and reproducibility characteristics for 6 replicate samples of 5 ug per litre were 2.5 and 4.7 per cent. 

chromatography; fluorescence; water analysis; analytical methods; pollutant identification; water pollution; pollution detection; liquid chromatography; nitrites; chemical analysis 

19TH INTERNATIONAL SYMP ON CHROMATOGRAPHY 

AIX EN PROVENCE, FRANCE