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1631534 
Journal Article 
Accelerated solvent extraction of lipids for determining the fatty acid composition of biological material 
Schafer, K 
1998 
Yes 
Analytica Chimica Acta
ISSN: 0003-2670
EISSN: 1873-4324 
ELSEVIER SCIENCE BV 
AMSTERDAM 
358 
1 
69-77 
English 
WOS:000071571800009 
Methods currently used for the quantitative determination
of total lipids and fatty acid composition in plant and animal tissues require solvent
extraction. An accelerated solvent-extraction (ASE) system and a modified Folch procedure were
compared in their ability to extract lipids from cereal, egg yolk and chicken breast muscle
samples. Fatty acid contents and compositions of extracted lipids were determined by gas-liquid
chromatography. Results varied with different extraction conditions. The fatty acid contents of
cereal and yolk lipids extracted by ASE were highest when using isopropanol-hexane (2:3, v/v),
however, the extraction of muscle lipids resulted in higher fatty acid contents when chloroform-
methanol (2:1, v/v) is used. Results indicate that ASE is a promising lipid-extraction system for
the entire range of plant and animal tissues. (C) 1998 Elsevier Science B.V. 
extraction; accelerated solvent extraction; solvent; lipids; fatty acids