US EPA

1651583 

Journal Article 

[Determination of nitric oxide in serum and plasma of human blood. Method of high pressure liquid chromatography] 

Kobylianskiĭ, AG; Kuznetsova, TV; Soboleva, GN; Bondarenko, ON; Pogorelova, OA; Titov, VN; Masenko, VP 

2003 

Biomedit︠s︡inskai︠a︡ khimii︠a︡
ISSN: 2310-6972
EISSN: 2310-6905 

49 

6 

597-603 

Russian 

16119088 

Two methods of measurement of nitrite and nitrate determination in human serum and plasma (a colorimetric method based on Griess reaction and a HPLC method) have been compared HPLC method revealed extremely low level of nitrites blood plasma, which was roughly the same (about 3 microM or less) in healthy donors, patients with diabetes and hypertension. The level of nitrates in the group of healthy donors (29.9 +/- 2.8 microM) is lower than in patients with diabetes (58.6 +/- 6.9 microM), and in patients with hypertension (35.4 +/- 2.5 microM). Comparison of data obtained by colorimetric and HPLC methods revealed close correlation (r = 0.77, p < 0.001). Methodical requirements for preparation of samples of serum and plasma of blood for analysis and correct intepretation of results are discussed in view of biochemical properties of nitric oxide molecule.