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HERO ID
1653309
Reference Type
Journal Article
Title
C-reactive protein attenuates endothelial progenitor cell survival, differentiation, and function: further evidence of a mechanistic link between C-reactive protein and cardiovascular disease
Author(s)
Verma, S; Kuliszewski, MA; Li, SH; Szmitko, PE; Zucco, L; Wang, CH; Badiwala, MV; Mickle, DA; Weisel, RD; Fedak, PW; Stewart, DJ; Kutryk, MJ
Year
2004
Is Peer Reviewed?
Yes
Journal
Circulation
ISSN:
0009-7322
EISSN:
1524-4539
Volume
109
Issue
17
Page Numbers
2058-2067
Language
English
PMID
15078802
DOI
10.1161/01.CIR.0000127577.63323.24
Web of Science Id
WOS:000221173900005
Abstract
BACKGROUND:
Myocardial ischemia provides a potent stimulus to angiogenesis, and the mobilization and differentiation of endothelial progenitor cells (EPCs) has been shown to be important in this process. An elevated level of C-reactive protein (CRP) has emerged as one of the most powerful predictors of cardiovascular disease. However, the impact of CRP on EPC biology is unknown.
METHODS AND RESULTS:
EPCs were isolated from the peripheral venous blood of healthy male volunteers. Cells were cultured in endothelial cell basal medium-2 in the absence and presence of CRP (5 to 20 microg/mL), rosiglitazone (1 micromol/L), and/or vascular endothelial growth factor. EPC differentiation, survival, and function were assayed. CRP at concentrations > or =15 microg/mL significantly reduced EPC cell number, inhibited the expression of the endothelial cell-specific markers Tie-2, EC-lectin, and VE-cadherin, significantly increased EPC apoptosis, and impaired EPC-induced angiogenesis. EPC-induced angiogenesis was dependent on the presence of nitric oxide, and CRP treatment caused a decrease in endothelial nitric oxide synthase mRNA expression by EPCs. However, all of these detrimental CRP-mediated effects on EPCs were attenuated by pretreatment with rosiglitazone, a peroxisome proliferator-activated receptor-gamma (PPARgamma) agonist.
CONCLUSIONS:
Human recombinant CRP, at concentrations known to predict adverse vascular outcomes, directly inhibits EPC differentiation, survival, and function, key components of angiogenesis and the response to chronic ischemia. This occurs in part via an effect of CRP to reduce EPC eNOS expression. The PPARgamma agonist rosiglitazone inhibits the negative effects of CRP on EPC biology. The ability of CRP to inhibit EPC differentiation and survival may represent an important mechanism that further links inflammation to cardiovascular disease.
Keywords
C-reactive protein; cells; apoptosis; nitric oxide synthase
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