US EPA

1682114 

Journal Article 

Adaptation of the Griess reaction for detection of nitrite in human plasma 

Giustarini, D; Dalle-Donne, I; Colombo, R; Milzani, A; Rossi, R 

2004 

Yes 

Free Radical Research
ISSN: 1071-5762
EISSN: 1029-2470 

38 

11 

1235-1240 

English 

15621701 

10.1080/10715760400017327 

WOS:000225877600012 

The determination of nitrite in human plasma or serum has been most frequently used as a marker of nitric oxide ( NO) production. In addition, it has recently been suggested that nitrite could act as a vasodilating agent at physiological concentrations by NO delivery. Therefore, nitrite determination in biological fluids is becoming increasingly important. The most frequently used method to measure nitrite is based on the spectrophotometric analysis of the azo dye obtained after reaction with the Griess reagent. This method has some limitations regarding detection limit and sensitivity, thus resulting unsuitable for nitrite detection in plasma. We have identified some drawbacks and modified the original procedure to overcome these problems. By the use of the newly developed method, we measured 221 +/- 72 nM nitrite in human plasma from healthy donors. 

nitrite; nitric oxide; Griess reaction; human plasma