US EPA

1682279 

Journal Article 

Hypochlorous acid and human blood low density lipoproteins modified by hypochlorous acid increase erythrocyte adhesion to endothelial cells 

Gorbatenkova, EA; Artmann, GM; Panasenko, OM 

1999 

Yes 

Biologicheskie Membrany
ISSN: 0233-4755 

16 

4 

437-444 

WOS:000083389400006 

The ability of hypochlorous acis (HOCl) (anion form - hypochlorite, OCl-) and HOCl/OCl- modified human blood low density lipoproteins (HOCl-LDLs) to stimulate erythrocyte adhesion to endothelial cell monolayers was studied. LDLs were modified by incubating at different HOCl/OCl- concentrations, This lead to a damage of proteins and lipids. We found (1) a more than 20-fold decrease of LDL fluorescence intensity (exinction at 285 nm. emission at 340 nm), (2) accumulation of secondary (TBA-reactive substances) and final (Schiff bases) products of lipid peroxidation, and (3) increase in the electrophoretic mobility of LDL. Preincubation of endothelial cells (ECs) with HOCl/OCl- (up to 50 mu M) enhanced erythrocyte adhesion to the EC monolayer. Preincubation of ECs with HOCl-LDLs (up to 250 mu M of HOCl/OCl- during LDL modification) (1) caused an increase in the cholesterol/phosplolipid molar ratio in ECs and (2) enhanced adhesion of erythrocytes to the endothelium. Application of HOCl/OCl- at concentrations above 50 mu M or treatment of LDLs with 500 mu M HOCl resulted in the cytotoxic effect on ECs and led to decreases in the molar cholesterol/phospholipid ratio in ECs and adhesion of erythocytes to the endothelium. The results suggest that HOCl/OCl- at physiological concentrations stimulates the adhesion of blood cells to the endothelium and cholesterol accumulation in the Vessel wall ECs either directly or due to LDL modification. Both effects might be important in the development of many vascular diseases.