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1749067 
Technical Report 
Transformation of o-toluate in Pseudomonas putida isolate 1065 and Rhizopus japonicus ATCC 24792 
Engelhardt, G; Wallnoefer, P 
1973 
PESTAB/74/0629 
Mikrobiol 
3 
PESTAB Pseudomonas putida isolate 1065 and Rhizopus japonicus ATCC 24794 were the only microorganisms of 36 isolated from soil capable of degrading o-toluate and 2-chlorobenzoate to a lesser extent. These compounds are products of the hydrolysis of systemic fungicides o-toluanilide and 2-chlorobenzanilide. The principal metabolites of o-toluate were 2-hydroxymethylbenzoic acid in the case of P. putida and 2-hydroxymethyltoluene in the case of R. japonicus. Phthalide was also isolated from the medium of P. putida. Conversion by P. putida. began in the stationary phase of growth while reduction by R. japonicus started in the early exponential phase of growth.