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1751790 
Journal Article 
A Simple Spectrophotometric Method for the Determination of Acetaldehyde in Blood 
Burbridge, TN; Hine, CH; Schick, AF 
1950 
Yes 
Journal of Laboratory and Clinical Medicine
ISSN: 0022-2143
EISSN: 1532-6543 
NIOSH/00128017 
35 
6 
983-987 
English 
15422240 
A procedure for the simultaneous handling of a number of acetaldehyde (75070) determinations was developed. Three milliliters (ml) of buffered semicarbazide hydrochloric-acid solution were pipetted into the center chamber of a diffusion cell. Two milliliters each of .66 normal sulfuric-acid and 10 percent sodium-tungstate were added to the outer chamber, and 2ml of fresh blood were then introduced to the outer chamber. After mixing, the cells were placed in an oven for 90 minutes at a temperature of 28 to 30 degrees-C to allow diffusion. Contents of the center cell were then removed, analyzed with a spectrophotometer, and compared to reference blanks. When the procedure was experimentally demonstrated, it was found that a 2ml blood specimen could be analyzed for acetaldehyde concentrations as low as 0.2 micrograms per ml with an accuracy of about 3.5 percent. The authors conclude that the method is particularly useful when a large number of determinations are to be made simultaneously. 
DCN-116415; Laboratory testing; Analytical processes; Analytical methods; Analytical chemistry; Chemical analysis; Blood analysis; Blood tests; Laboratory techniques