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1760017 
Journal Article 
Activated aluminum oxide selectively retaining long chain n-alkanes: Part II. Integration into an on-line high performance liquid chromatography-liquid chromatography-gas chromatography-flame ionization detection method to remove plant paraffins for the determination of mineral paraffins in foods and environmental samples 
Fiselier, K; Fiorini, D; Grob, K 
2009 
Yes 
Analytica Chimica Acta
ISSN: 0003-2670
EISSN: 1873-4324 
Elsevier Science BV, P.O. Box 211, Amsterdam, 1000 AE, Netherlands, [mailto:w.tukker@elsevier.nl], [URL:http://www.elsevier.com] 
Netherlands 
634 
1 
102-109 
English 
19154817 
WOS:000263189500015 
Aluminum oxide activated by heating to 300-400 degrees C retains n-alkanes with more than about 20 carbon atoms, whereas iso-alkanes largely pass non-retained (with characteristics described in more detail in Part I). This property is useful for the analysis of mineral oil contamination of foods and other matrices: it enables the removal of plant n-alkanes, typically ranging from C(23) to C(33), when they disturb the analysis of mineral paraffins (usually almost exclusively consisting of iso-alkanes). An on-line HPLC-LC-GC-FID method is proposed in which a first silica gel HPLC column isolates the paraffins from the bulk of edible oils or extracts and is backflushed with dichloromethane. In a second separation step, a 10 cm x 2 mm i.d. column packed with activated aluminum oxide separates the long chain n-alkanes from the fraction of the iso-alkanes which is transferred to GC-FID by the on-column interface and the retention gap technique. The retained n-alkanes are removed by flushing with iso-octane. 
Activated aluminum oxide; Retention of long chain n-alkanes; Determination of mineral paraffins in foods; On-line high performance liquid chromatography-liquid chromatography-gas chromatography-flame ionization detection