US EPA

1762182 

Journal Article 

The Cytotoxic and Ultrastrctural Perturbations of Aluminum Exposed Nile Catfish with Special Reference to the Mitigating Effect of Vitamin C 

Moustafa, GG; Khalil, S; Hussein, MMA; Zeinab, ML 

2012 

1 

Life Science Journal
ISSN: 1097-8135 

9 

4 

5198-5210 

WOS:000316686000150 

The genotoxic parameters are currently among the most
valuable fish biomarkers for environmental risk assessment. So, the present study was directed to
explore the toxic impacts of Aluminum chloride (AlCl3) on DNA damage, apoptosis, antioxidant
status and ultrastructural investigations of Nile catfish. The experiment was carried out on 48
fish that randomly divided into four equal groups with two replicates; the first group exposed to
1/20 LC50 of Al//www.lifesciencesite.com, the second group exposed to the combined doses of AlCl3
(1/20 LC50) and vitamin C (Vit. C) at dose of 5 ppm, the third group exposed to Vit C at the dose
previously mentioned and the fourth group was kept as negative control. The experiment was
terminated after six months where the fish were sacrified and specimens from liver and gills of
all groups were obtained and kept at -20 degrees C till applying the required measurements and
another specimen from the same organs were fixed in 10% neutral -buffered formalin and 3%
glutaraldehyde solution for histopathological examination. The results indicated pronounced
significant increase in malondialdehyde (MDA) concentration and significant decrease in both
reduced glutathione (GSH) concentration and superoxide dismutase (SOD) and catalase (CAT) enzyme
activity. AlCl3 elicited an obvious increase in oxidative DNA damage and frequency of apoptotic
cells, these manifestation were markedly ameliorated in the group exposed to the combined doses
of AlCl3 and Vit C. The ultrastructural histopathological findings proved the aforementioned
results. It could be concluded that AlCl3 elucidated a marked ruinous effects on the oxidative
and genotoxic impacts as well as the histopathological alterations which were alleviated by
Vitamin C. [Gihan G. Moustafa, Samah Khalil; Mohamed M. A. Hussein and Zeinab, M. Labib The
Cytotoxic and Ultrastrctural Perturbations of Aluminum Exposed Fish with Special Reference to the
Mitigating Effect of Vitamin C] Life Sci J 2012; 9(4):5198-5210]. (ISSN:1097-8135).
http://www.lifesciencesite.com. 773 

Aluminum chloride; Apoptosis; DNA damage; Oxidative stress; Ultrastructural investigations; Vitamin C