Method to Determine British Anti-Lewisite in Plasma Utilizing Gas Chromatography/Mass Spectrometry | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

1784815

Reference Type

Technical Report

Title

Method to Determine British Anti-Lewisite in Plasma Utilizing Gas Chromatography/Mass Spectrometry

Author(s)

Byers, CE; Holloway, ER; Korte, WD; Smith, JR; Capacio, BR

Year

2005

Report Number

NTIS/02941963_a

Volume

GRA and I

Issue

GRA and I

Abstract

British anti-Lewisite (BAL) (2,3-dimercapto-1-propanol) is a potential therapeutic compound against the effects of cutaneous sulfur mustard, and an method for its determination in plasma has been developed. BAL and the internal standard (IS) ethane dithiol were removed from plasma samples through solid phase extraction, then reacted with 1-pentafluoropropionylimidazole (PFPI), forming stable pentafluoropropionyl derivates that are sensitive to gas chromatography/mass spectroscopy (GC/MS) analysis. Examining concentrations versus peak area ratios of BAL and IS derivatives demonstrated the method to be linear from 0.48 to 124 ng/ml in plasma with acceptable accuracy and precision. This method has proven to be both a reliable and sensitive means to determine BAL in plasma.

Keywords

Therapy; *Mustard agents; Organometallic compounds; Detoxification; Lewisite; Chelating agents; Blood analysis; Blood plasma; Gas chromatography; Mass spectroscopy; Sulfur mustard; British antilewisite