US EPA

1785288 

Journal Article 

Inhibition By Aminoacetonitrile Of Allylamine-Induced Lesions In The Rat's Liver 

Fiume, L 

1962 

Yes 

Journal of Pathology and Bacteriology
ISSN: 0368-3494 

NIOSH/00142976 

84 

256-258 

The effect of aminoacetonitrile (540614) (AAN) on allylamine (107119) induced liver lesions was studied in rats. Thirty male rats were injected intraperitoneally for 3 consecutive days with 0.01 milliliters (ml) allylamine dissolved in 0.2ml water and neutralized with sulfuric-acid; 30 rats received allylamine alone. Some rats also received a daily subcutaneous injection of 20 milligrams AAN (as the hydrosulfate salt) dissolved in 0.2ml water and neutralized with sodium-hydroxide. This treatment was administered 4 days prior to allylamine administration and for the 3 days of allylamine administration. During the experiment, four rats died in the group treated with allylamine alone; no rats treated with allylamine plus AAN died. Twenty four hours after the last allylamine injection, all animals were killed. Sections of liver were fixed in 4 percent formaldehyde and stained with hematoxylin and eosin. The livers of all rats treated with allylamine alone showed severe alterations of the parenchymal cells in all areas of the hepatic lobule. The cells appeared remarkably swollen, with optically empty cytoplasm, or were reduced to large vesicles. These alterations were reduced or absent in the livers of all the rats treated with allylamine plus AAN. The author suggests that, because allylamine is known to increase the permeability of hepatic sinusoids, AAN may exert its effect by partly or completely inhibiting allylamine induced permeability changes. 

DCN-130478; Biochemical analysis; Physiological measurements; Toxicology; Medical research; Medical monitoring; Liver disorders; Animal studies; Laboratory testing; Clinical techniques