Dissection of the maturation reactions of the [NiFe] hydrogenase 3 from Escherichia coli taking place after nickel incorporation | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

1796685

Reference Type

Journal Article

Title

Dissection of the maturation reactions of the [NiFe] hydrogenase 3 from Escherichia coli taking place after nickel incorporation

Author(s)

Magalon, A; Böck, A

Year

2000

Is Peer Reviewed?

Yes

Journal

FEBS Letters
ISSN: 0014-5793
EISSN: 1873-3468

Volume

473

Issue

Page Numbers

254-258

Language

English

PMID

10812085

Web of Science Id

WOS:000087126800028

Abstract

The steps in the maturation of the precursor of the large subunit (pre-HycE) of hydrogenase 3 from Escherichia coli taking place after incorporation of both iron and nickel were investigated. Pre-HycE could be matured and processed in the absence of the small subunit but association with the cytoplasmic membrane required heterodimer formation between the two subunits. Pre-HycE formed a complex with the chaperone-like protein HypC in the absence of the small subunit and, in this complex, also incorporated nickel. For the C-terminal processing, HypC had to leave the complex since only a HypC-free, nickel-containing form of pre-HycE was a substrate for the maturation endopeptidase.

Keywords

[NiFe] hydrogenase; maturation; cellular location; Escherichia coli