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1797276 
Journal Article 
Low molecular weight targets of metals in human kidney 
Templeton, DM; Sarkar, B 
1986 
Yes 
Acta Pharmacologica et Toxicologica
ISSN: 0001-6683 
NIOSH/00166419 
59 Suppl 7 
7 
416-423 
English 
3776599 
The ligands of ionic nickel (7440020) (Ni) in the kidney soluble fraction were characterized in order to identify the two forms of the metal responsible for its rapid excretion and the potential carcinogenic complexes of intracellular Ni. Exposure of human kidney cytosol to radioactive labelled nickel-chloride (7718549) revealed that the low molecular weight Ni binding fraction consisted of two Ni binding components which were separated by gel exclusion high performance liquid chromatography and accounted for two thirds of the soluble renal Ni. One of the components was found to bind 70 percent of the Ni and consisted of anionic oligosaccharides having typical oligosaccharide and glycosaminoglycan chemical compositions. The other component was an acidic peptide having an amino acid composition consistent with a molecular weight of 4 kilodaltons, with no aromatic amino acids. Tests to determine the binding of other metals to the two newly identified Ni binding components showed binding of both copper (7440508) (to oligosaccharides) and cadmium (7440439) (to peptide). The authors conclude that the presence of peptide and carbohydrate complexes in kidney cytosol could be significant in regard to the intracellular redistribution of the metal, and that their further study could clarify features of metal induced nephrotoxicity.