US EPA

1812887 

Journal Article 

HPLC Analysis of Oxidation Hair Dyes in Permanent Hair Colorants 

Andrisano, V; Gotti, R; Di Pietra, AM; Cavrini, V 

1994 

Yes 

Journal of Liquid Chromatography
ISSN: 0148-3919 

NIOSH/00222731 

17 

13 

2919-2937 

A liquid chromatographic technique for determining oxidant dyes in commercial hair coloring formulations was developed. Aliquots of 0.2 grams hair colorant paste were dispersed in a solution containing 0.85% orthophosphoric-acid and 0.5% sodium-sulfite in a 100 milliliter (ml) volumetric flask using magnetic stirring. After filtering, the filtrate was applied to an SPE cartridge containing SCX sorbent. The analyte was eluted with a solution containing 0.25 molar (M) phosphate buffer (pH 8.0), 1M sodium-chloride, 5M urea, and 0.5% sodium-sulfite. The eluates were acidified with 200 microliters (microl) 17% orthophosphoric-acid. 2-Nitro-1,4-phenylenediamine was added as the internal standard. Aliquots of 20microl were injected into a high performance liquid chromatography system fitted with a photodiode detector. The mobile phase consisted of binary mixtures of acetonitrile and a pH 4.5 buffer containing 0.005M 1,8-diaminooctane (DAO) and 0.020M heptanesulfonate. The acetonitrile content of the mobile phase varied from 5 to 30%. The basic components retained on the SPE column were eluted with a phosphate buffer (pH 8.0) containing 1M sodium-chloride and 5M urea. After acidification with 0.85% orthophosphoric-acid they were analyzed as before. Representative chromatograms of mixtures of the components of 14 dye formulations showed good separation. Calibration plots for dye components such as m-aminophenol (591275) (MAP), p-aminophenol (123308) (PAP), 1,4-phenylenediamine (106503) (PPDA), and 4-amino-o-cresol (2835963) were linear over the ranges 1.0 to 20.0, 1.0 to 23.0, 1.5 to 25.0, and 1.5 to 12.0 micrograms per milliliter, respectively. The procedure was applied to determining the concentrations of the components in commercial hair colorants and checking the results against data supplied by the manufacturers. Representative data indicated that the concentrations of PPDA, PAP, and MAP measured by the procedure averaged 80.08 to 103.30% of the concentrations reported by the manufacturers. The authors conclude that the procedure provides adequate selectivity for determining the most important oxidant hair dyes. 

DCN-222427; Analytical methods; Amino compounds; Phenolic compounds; Cosmetics; Coloring materials; Sample preparation; Chemical extraction; Chemical composition