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1849970 
Journal Article 
Generating tamoxifen-inducible Cre alleles to investigate myogenesis in mice 
Lepper, C; Fan, CM 
2012 
1 
Methods in Molecular Biology
ISSN: 1064-3745
EISSN: 1940-6029 
HUMANA PRESS INC 
TOTOWA 
MYOGENESIS: METHODS AND PROTOCOLS 
798 
297-308 
English 
22130844 
WOS:000299298100017 
Gene inactivation has become the gold standard for determining gene function in the mouse. Many genes inactivated in the germ line cause early lethality that precludes phenotypic assessment at a later time point. Conditional gene inactivation using Cre recombinase expressed via a tissue specific promoter/enhancer allows phenotypic analyses of selected tissues, but lacks temporal control. Recent development of the tamoxifen-inducible Cre-ER (T2) offers both cell type-specific and temporal control of conditional gene inactivation. As an example, we describe the design and step-wise construction of a Cre-ER (T2) knock-in allele at the Pax7 locus using the recombineering method - Pax7 is selectively expressed in embryonic muscle progenitors and adult muscle stem cells. The resulting Pax7-Cre- ER (T2) (Pax7 (CE)) allele has been successfully applied to embryos and adults for tamoxifen-regulated myogenic lineage tracing and gene inactivation (Nature 460:627-631, 2009; Genesis 48:424-436, 2010). 
DiMario, JX; 
978-1-61779-342-4