Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
1851227
Reference Type
Journal Article
Title
Functional analysis of the interaction between the mismatch repair protein MutS and the replication processivity factor β clamp in Pseudomonas aeruginosa
Author(s)
Monti, MR; Miguel, V; Borgogno, MV; Argaraña, CE
Year
2012
Is Peer Reviewed?
Yes
Journal
DNA Repair
ISSN:
1568-7864
EISSN:
1568-7856
Volume
11
Issue
5
Page Numbers
463-469
Language
English
PMID
22365420
DOI
10.1016/j.dnarep.2012.01.015
Abstract
Interaction between MutS and the replication factor β clamp has been extensively studied in a Mismatch Repair context; however, its functional consequences are not well understood. We have analyzed the role of the MutS-β clamp interaction in Pseudomonas aeruginosa by characterizing a β clamp binding motif mutant, denominated MutSβ, which does not interact with the replication factor. A detailed characterization of P. aeruginosa strain PAO1 harboring a chromosomal mutSβ allele demonstrated that this mutant strain exhibited mutation rates to rifampicin and ciprofloxacin resistance comparable to that of the parental strain. mutSβ PAO1 was as proficient as the parental strain for DNA repair under highly mutagenic conditions imposed by the adenine base analog 2-aminopurine. In addition, using a tetracycline resistance reversion assay to assess the repair of a frameshift mutation, we determined that the parental and mutSβ strains exhibited similar reversion rates. Our results clearly indicate that the MutS-β clamp interaction does not have a central role in the methylation-independent Mismatch Repair of P. aeruginosa.
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity