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1855604 
Journal Article 
Hydrogenase in Frankia KB5: Expression of and relation to nitrogenase 
Mattsson, U; Sellstedt, A 
2000 
Yes 
Canadian Journal of Microbiology
ISSN: 0008-4166
EISSN: 1480-3275 
46 
12 
1091-1095 
English 
11142397 
WOS:000165676800001 
The localization and expression of the hydrogenase in free-living Frankia KB5 was investigated immunologically and by monitoring activity, focusing on its relationships with nitrogenase and H2. Immunological studies revealed that the large subunit of the hydrogenase in Frankia KB5 was modified post-translationally, and transferred into the membrane after processing. The large subunit was constitutively expressed and no correlation was found between hydrogenase activity and synthesis. Although H2 was not needed for induction of hydrogenase synthesis, exogenously added H2 triggered hydrogen uptake in medium containing nitrogen, i.e., in the hyphae. A correlation between nitrogenase activity and hydrogen uptake was found in cultures grown in media without nitrogen, but interestingly the two enzymes showed no co-regulation. 
hydrogenase; expression; nitrogenase; Frankia KB5