US EPA

1860965 

Journal Article 

Separation and enrichment of zinc, copper, iron, cadmium, cobalt, and nickel from urine with Amberlite XAD-16 resin 

Soylak, M; Dogan, M 

1996 

1 

Trace Elements and Electrolytes
ISSN: 0946-2104 

NIOSH/00235650 

13 

3 

130-132 

WOS:A1996UY62300006 

An effort was made to determine trace levels of iron (7439896) (Fe), nickel (7440020) (Ni), copper (7440508) (Cu), zinc (7440666) (Zn), cadmium (7440439) (Cd), and cobalt (7440484) (Co) in urine using a separation and preconcentration procedure. Prior to using urine samples, model solutions were used in the Amberlite-XAD-16-resin column procedure for the preconcentration and separation of heavy metal ions. Metal concentrations in the final solution were determined by flame atomic absorption spectrometry (FAAS) and graphite furnace atomic absorption spectrometry (GFAAS). After obtaining reference and working solutions, urine samples were acid digested and pH buffered prior to separation and enrichment of trace metal ions and spectrometric analysis. The enrichment procedure involved the addition of a chelating agent (sodium-diethyldithiocarbamate) to the sample solutions prior to recovery using the resin column. FAAS was used to determine Zn and Fe, while GFAAS was used to determine Cd, Cu, Ni, and Co in the final solutions. Quantitative recoveries at 95% or above were found to be in the pH 4 to 8 range, with the optimum pH of 4 for quantitative recoveries of all working metal ions. Matrix ions in urine did not affect trace metal recoveries. The relative standard deviations of the preconcentration procedure ranged between 0.05 and 0.07. Values for the added and detected analyte content were in good agreement. The authors conclude that the separation/preconcentration procedure provides a precise and accurate method for determining trace amounts of heavy metals in the urine and may be applied to other biological samples such as blood and serum. 

Amberlite XADII6; enrichment; separation; trace metal ions; urine