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1862016 
Journal Article 
Generation of free-radicals in reactions of Ni(II)-thiol complexes with molecular-oxygen and model lipid hydroperoxides 
Shi, X; Dalal, NS; Kasprzak, KS 
1993 
Yes 
Journal of Inorganic Biochemistry
ISSN: 0162-0134
EISSN: 1873-3344 
50 
3 
211-225 
English 
8388916 
WOS:A1993LA47700005 
BIOSIS COPYRIGHT: BIOL ABS. The generation of free radicals from reactions of nickel(II)-thiol complexes with molecular oxygen and model lipid hydroperoxides was investigated by electron spin resonance (ESR) utilizing 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) as a spin trap. Incubation of nickel(II) (Ni(II)) with cysteine in an aerobic environment generated hydroxyl ( OH) radical, which then reacted with cysteine to generate a carbon-centered alkyl ( R) radical. Radical generation was inhibited under a nitrogen atmosphere. Model lipid hydroperoxides, cumene hydroperoxide, and t-butyl hydroperoxide enhanced the yield of these radicals and also generated an alkoxyl ( OR) radical. Radical yield decreased by approximately half under a nitrogen atmosphere. Although histidine did not cause radical formation in the reaction between Ni(II) and cumene hydroperoxide under aerobic conditions, the addition of histidine to a mixture containing Ni(II), cysteine, and cumene hydroperoxide under the same experimenta