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1862392 
Journal Article 
BIOTIN DEXTRAN - A SENSITIVE ANTEROGRADE TRACER FOR NEUROANATOMICAL STUDIES IN RAT AND MONKEY 
Brandt, HM; Apkarian, AV 
1992 
Yes 
Journal of Neuroscience Methods
ISSN: 0165-0270 
Elsevier 
45 
1-2 
35-40 
English 
1283432 
WOS:A1992KD14400004 
The properties of a new anterograde tracer, biotin-dextran (BD), are reported. Iontophoretic or pressure injections of BD in the spinal cord of rats and monkeys revealed terminal-like BD label in many regions of the brain. The BD label was visualized by an avidin-biotin reaction combined with diaminobenzidine, with and without nickel enhancement. This reaction resulted in permanent label and revealed the fine morphology of terminal boutons and en-passant endings. Biotin-dextran is an excellent anterograde tracer that can also be visualized at the electron microscopic level, used in combination with other retrograde and anterograde tracers and with immunohistochemical labeling of neurotransmitters. 
BIOTIN; DEXTRAN; RETROGRADE LABEL; AXONAL LABEL; DIAMINOBENZIDINE; SPINAL CORD