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1862529 
Journal Article 
DIFFERENTIAL VISUALIZATION OF CHOLINESTERASIC NEURONAL SOMATA AND FIBERS BY USE OF MODIFICATIONS OF ACETYLCHOLINESTERASE PHARMACOHISTOCHEMISTRY 
Dipatre, PL; Mathes, CW; Butcher, LL 
1993 
Yes 
Journal of Histochemistry and Cytochemistry
ISSN: 0022-1554
EISSN: 1551-5044 
41 
1 
129-135 
English 
7678024 
WOS:A1993KD78200018 
We modified existing acetylcholinesterase (AChE) histochemical techniques to visualize cholinergic neuronal somata and processes in the same rat brain. The AChE staining procedure of Karnovsky and Roots, combined with diisopropylfluorophosphate (DFP) pre-treatment, have previously allowed visualization of neuronal somata. Fibers have been visualized by nickel-diaminobenzidine (DAB)-hydrogen peroxide histochemical intensification. We combined novel modifications of these techniques to visualize cell bodies and fibers simultaneously. Maximal staining of neuronal somata occurs with a 1:1 dilution of both the Karnovsky-Roots medium and the nickel-DAB solution; diluting the Karnovsky-Roots medium 1:10 and increasing the concentration of the nickel-DAB solution twofold allows visualization of fibers previously unstainable with DFP pre-treatment and the classical AChE protocol. It is now possible to visualize both neuronal somata and fibers in adjacent sections of the same brain, in a quick and inexpensive manner. 
ACETYLCHOLINESTERASE (ACHE); DIISOPROPYLFLUOROPHOSPHATE (DFP); DIAMINOBENZIDINE (DAB)