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Citation
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HERO ID
1928172
Reference Type
Journal Article
Title
Enhanced Biodegradation of Oil Products by Some Microbial Isolate Supplemented with Heavy Metals
Author(s)
Sarma, A; Sarma, H
Year
2010
Is Peer Reviewed?
Yes
Journal
International Journal of Botany
ISSN:
1811-9700
EISSN:
1811-9719
Volume
6
Issue
4
Page Numbers
441-448
Language
English
DOI
10.3923/ijb.2010.441.448
URL
http://search.proquest.com/docview/904476172?accountid=102841
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Abstract
Three native strains, identified as Staphylococcus sp., Acinetobactor Iwoffii and Enterobacter agglomerans were isolated from crude oil contaminated field and the aim of this research was to select potential soil microbial strain that could be effective in the bioremediation of crude oil compounds. Crude oil degradation performed by the isolates incubated in the shake flask culture and analysis of the results address that Acinetobactor Iwoffii, accelerated cleanup most effectively, degrading oil sludge by a total of 93.78% in compare to Staphylococcus sp. (17.39%) and Enterobacter agglomerans (16.49%). Furthermore enhanced biodegradation potential of the isolates were studied by adding Mn and Cu and results indicated that supplemented metal increased degradation of crude oil products. In the present study, three bacterial strains AS1, AS2 and AS3 were termed and isolated through long cultivation with crude oil as the single carbon source. The three strains were identified based on the morphology of their colonies with physiological and biochemical characteristics. In addition, the characterizations of soil where the presence of these strains were carried out. The total CFU count ranged from 2x10 super(6) to 6x10 super(6) and total viable bacteria at 32 degree C ranged from 2.2x10 super(3) to 5.6x10 super(3) in various soil sample collected in random from contaminated site. The strains had broad degradation capacities and the present remediation monitoring confirmed the effectiveness of Acinetobactor Iwoffii has one of the potential native microbes for remediation of crude oil soil.
Keywords
Microbiology Abstracts A: Industrial & Applied Microbiology; Toxicology Abstracts; Biotechnology and Bioengineering Abstracts; Bioremediation; Biodegradation; Sludges; Heavy metals; Staphylococcus; Cell culture; Carbon sources; Colonies; Biochemical characteristics; Colony-forming cells; Enterobacter agglomerans; Cadmium; Manganese; W 30950:Waste Treatment & Pollution Clean-up; A 01450:Environmental Pollution & Waste Treatment; X 24360:Metals
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