Health & Environmental Research Online (HERO)


Print Feedback Export to File
1944092 
Journal Article 
Biodegradation of trichloroethylene by Methylosinus trichosporium OB3b 
Tsien, HC; Brusseau, GA; Hanson, RS; Waclett, LP 
1989 
Yes 
Applied and Environmental Microbiology
ISSN: 0099-2240
EISSN: 1098-5336 
BIOSIS/90/03991 
55 
12 
3155-3161 
English 
2515801 
WOS:A1989CC66600021 
The methanotroph Methylosinus trichosporium OB3b, a type II methanotroph, degraded trichloroethylene at rates exceeding 1.2 mmol/h per g (dry weight) following the appearance of soluble methane monooxygenase in continuous and batch cultures. Cells capable oxidizing trichloroethylene contained components of soluble methane monooxygenase as demonstrated by Western blot (immunoblot) analysis with antibodies prepared against the purified enzyme. Growth of cultures in a medium containing 0.25 microM or less copper sulfate caused derepression of the synthesis of soluble methane monooxygenase. In these cultures, the specific rates of methane and methanol oxidation did not change during growth, while trichloroethylene oxidation increased with the appearance of soluble methane monooxygenase. M. trichosporium OB3b cells that contained soluble methane monooxygenase also degraded vinyl chloride, 1,1-dichloroethylene, cis-1,2-dichloroethylene, and trans-1,2-dichloroethylene. 
BIOCHEMISTRY/METHODS; BIOCHEMISTRY; ENZYMES/ANALYSIS; ENZYMES/PHYSIOLOGY; METABOLISM; ENVIRONMENTAL POLLUTANTS/POISONING; OCCUPATIONAL DISEASES; BACTERIA/PHYSIOLOGY; BACTERIA/METABOLISM; SANITATION; AIR POLLUTION; SOIL POLLUTANTS; WATER POLLUTION; BIODEGRADATION; INDUSTRIAL MICROBIOLOGY; METHYLOCOCCACEAE; Biochemical Methods-General; Biochemical Studies-General; Enzymes-Methods; Enzymes-Physiological Studies; Metabolism-General Metabolism; Toxicology-Environmental and Industrial Toxicology; Physiology and Biochemistry of Bacteria; Public Health: Environmental Health-Sewage Disposal and Sanitary Measures; Public Health: Environmental Health-Air; Food and Industrial Microbiology-Biodegradation and Biodeterioration; Methylomonadaceae (1979- ); 156-60-5; 156-59-2; 79-01-6; 75-35-4; 75-01-4; 74-82-8