Health & Environmental Research Online (HERO)


Print Feedback Export to File
1965243 
Journal Article 
Electrosurface properties of poly(styrene-co-acrolein) latexes with the protein-modified surface 
Men'shikova, AY; Dmitrieva, IB; Kuchuk, VI; Skurkis, YO; Evseeva, TG; Shabsel's, BM 
1999 
Yes 
Colloid Journal
ISSN: 1061-933X
EISSN: 1608-3067 
Plenum Publ Corp 
61 
6 
740-748 
English 
WOS:000084318100010 
The effect of pH on the electrophoretic mobility of poly(styrene-co-acrolein) microspheres is studied. It is established that pH(IEP) is equal to 2.0. The abnormal dependence of the electrophoretic mobility of latex particles on NaCl concentration is obtained. It is shown that the exchange capacities of H+ and OH- ions are much lower than the surface concentrations of carboxyl and aldehyde groups, pH(PZC) being equal to 6.5. The isotherms of bovine serum albumin (BSA) adsorption on the microsphere surface exhibit a pattern typical of chemisorption; the bound BSA is almost completely responsible for the electrosurface properties of microspheres. The values of pH(IEP) fall within the range of 4.3-5.6; the passage from the native to denatured forms of BSA and a decrease in the amount of bound protein results in an increase in the pH(IEP) and the electrophoretic mobility of particles in the alkaline region of pH. The exchange capacity of the BSA-modified microsphere surface with respect to H+ and OH- ions markedly increases, and pH(PZC) is equal to 7.5. The results obtained are discussed in terms of the formation of the layer of polymer chains enriched with hydrophilic units on the latex particle surface and the conformational variations of these chains and BSA macromolecules under the action of a dispersion medium.