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1972982 
Journal Article 
Optimization and characteristics of preparing chitosan microspheres using response surface methodology 
Zhou, X; Cheng, X; Liu, W; Li, J; Ren, LiH; Dang, QiF; Feng, C; Chen, XiG 
2013 
Yes 
Journal of Applied Polymer Science
ISSN: 0021-8995
EISSN: 1097-4628 
127 
6 
4433-4439 
WOS:000312940400029 
In this study, response surface methodology (RSM) was employed to optimize preparation of chitosan microspheres (CMs). Firstly, PlackettBurman design (PBD) was applied to screen out the factors, which influence preparation of CMs significantly. The results showed that the concentration of chitosan and acetic acid as well as the volume of toluene were the key factors. Then, steepest ascent experiment and BoxBehnken design were introduced to optimize the levels of the key factors. As a result, the appropriate conditions of preparing CMs were chosen as follows: 2% (w/v) chitosan, 1.7% (v/v) acetic acid, 7 mL span-80, 488 mL toluene, 1100 rpm, 60 min (emulsification time), 10 mL formaldehyde, and 60 min (crosslinking time). Also, the morphology, size particle, and FTIR spectra of CMs were studied by scanning electron microscopy, small angle laser light scattering, and FTIR spectrometer. Results showed that CMs had quite smooth surface spherical and sharp size distribution, which indicated that the CMs were successfully prepared by W/O emulsification crosslinking method using RSM. (c) 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013 
chitosan; chitosan microspheres; response surface methodology; Plackett-Burman design; Box; Behnken design