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1973674 
Journal Article 
Formaldehyde-Based Whole-Mount In Situ Hybridization Method for Planarians 
Pearson, BJ; Eisenhoffer, GT; Gurley, KA; Rink, JC; Miller, DE; Alvarado, AS 
2009 
Yes 
Developmental Dynamics
ISSN: 1058-8388
EISSN: 1097-0177 
238 
2 
443-450 
English 
19161223 
WOS:000263229100020 
Whole-mount in situ hybridization (WISH) is a powerful tool for visualizing gene expression patterns in specific cell and tissue types. Each model organism presents its own unique set of challenges for achieving robust and reproducible staining with cellular resolution. Here, we describe a formaldehyde-based WISH method for the freshwater planarian Schmidtea mediterranea developed by systematically comparing and optimizing techniques for fixation, permeabilization, hybridization, and postprocessing. The new method gives robust, high-resolution labeling in fine anatomical detail, allows co-labeling with fluorescent probes, and is sufficiently sensitive to resolve the expression pattern of a microRNA in planarians. Our WISH methodology not only provides significant advancements over current protocols that make it a valuable asset for the planarian community, but should also find wide applicability in WISH methods used in other systems. 
in situ; whole-mount; planaria; mucolytics 
IRIS
• Formaldehyde [archived]
     Reproductive and Developmental Effects
          Screened
               Title/abstract
                    Irrelevant species
     Retroactive RIS import
          2015
               FA DevRepro 072115
                    Irrelevant Species-Population Criteria
• IRIS Formaldehyde (Inhalation) [Final 2024]
     Literature Indexing
          Other sources and cited references
     Literature Identification
          Reproductive and Developmental Effects
               Excluded