US EPA

1974646 

Journal Article 

Variations in tight and gap junctions in mammalian tissues 

Friend, DS; Gilula, NB 

1972 

Yes 

Journal of Cell Biology
ISSN: 0021-9525
EISSN: 1540-8140 

53 

3 

758-776 

English 

4337577 

10.1083/jcb.53.3.758 

The fine structure and distribution of tight (zonula occludens) and gap junctions in epithelia of the rat pancreas, liver, adrenal cortex, epididymis, and duodenum, and in smooth muscle were examined in paraformaldehyde-glutaraldehyde-fixed, tracer-permeated (K-pyroantimonate and lanthanum), and freeze-fractured tissue preparations. While many pentalaminar and septilaminar foci seen in thin-section and tracer preparations can be recognized as corresponding to well-characterized freeze-fracture images of tight and gap junction membrane modifications, many others cannot be unequivocally categorized-nor can all freeze-etched aggregates of membrane particles. Generally, epithelia of exocrine glands (pancreas and liver) have moderate-sized tight junctions and large gap junctions, with many of their gap junctions basal to the junctional complex. In contrast, the adrenal cortex, a ductless gland, may not have a tight junction but does possess large gap junctions. Mucosal epithelia (epididymis and intestine) have extensive tight junctions, but their gap junctions are not as well developed as those of glandular tissue. Smooth muscle contains numerous small gap junctions The incidence, size, and configuration of the junctions we observed correlate well with the known functions of the junctions and of the tissues where they are found. 

Adrenal Glands/cytology; Animals; Antimony; Duodenum/cytology; Epididymis/cytology; Epithelial Cells; Freeze Etching; Histological Techniques; Hyaluronoglucosaminidase; Intercellular Junctions; Intestinal Mucosa/cytology; Lanthanum; Liver/cytology; Microbial Collagenase; Microscopy, Electron; Muscle, Smooth/cytology; Pancreas/cytology; Rats, Inbred Strains