US EPA

1977738 

Journal Article 

Effects of monocular enucleation on the superior colliculus of adult rabbit-A light microscopic study 

Khan, MA; Khan, AA; Faruqi, NA 

2008 

1 

Biomedical Research
ISSN: 0970-938X
EISSN: 0976-1683 

19 

1 

27-31 

English 

WOS:000256665300007 

http://://WOS:000256665300007
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Monocular enucleation. induced changes in the visual system
in neonates have been reported by many workers but information regarding its effects in adults
remains scanty. Present study is an attempt to find out the latter in rabbits. 25 adult rabbits
of either sex were subjected to monocular enucleation under general anaesthesia. A similar number
of nonenucleates served as controls. Animals were sacrificed after post lesion-survival periods
ranging from 2 weeks to 4 months. Brains were fixed by trans-cardiac perfusion of 10% formalin.
Superior colliculi were removed from midbrain and processed for paraffin embedding. 10 1 1 thick
sections were stained with Haematoxylin and Eosin, Cresyl violet and Luxol fast blue and Thionin
as well as Kluver Barrera and Glees and Marshland stains. To access the deafferentation-induced
changes in the contralateral (with respect to enucleation), sections from superior colliculi were
observed under light microscope. Morphological changes were in the form of vacuolation in the
neuropil, fascicular disorganization and fragmentation of myelin, cytoplasmic and nuclear
disintegration of neurons and glial cell aggregation. It was concluded that though the
deafferentation-induced changes were not dramatic at any point of time during study, yet its
extent could be correlated with duration of postexperimental survival period of the animal. 

superior colliculus; monocular enucleation; anterograde degeneration