US EPA

1978183 

Journal Article 

Development of indirect competitive ELISA for the detection of Salmonella typhimurium 

Bang, J; Shukla, S; Kim, YH; Kim, M 

2012 

1 

Romanian Biotechnology Letters
ISSN: 1224-5984 

17 

2 

7194-7204 

WOS:000303544200017 

http://://WOS:000303544200017
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Due to increased rate of Salmonella infections in humans
and animals, a rapid indirect competitive enzyme-linked immunosorbent assay (IC-ELISA), was
developed in this study for routine analytical detection of Salmonella typhimurium. Formalin
killed whole cell lysate of S. typhimurium was subcutaneously injected as an antigen into rabbit
to produce antibody. The optimum production of rabbit anti-S. typhimurium immunoglobulin G (IgG)
was reached to the highest level on 8 week. A two step caprylic acid and ammonium sulfate
precipitation method was used to purify rabbit anti-S. typhimurium IgG from the immunized rabbit
sera. The purified IgG was used for the detection of S. typhimurium through IC-ELISA. The
developed technique was able to detect 10(6) cells/ml of S. typhimurium. Also, the specificity of
the proposed immunoassay was investigated with different foodborne pathogens including
Escherichia coli O157:H7, Cronobacter muytjensii, Enterobacter aerogenes and Salmonella
enteritidis which produced no significant reaction signal as compared with S. typhimurium. These
findings indicate that IC-ELISA could be used as an alternative method for detection of S.
typhimurium. 

anti-Salmonella immunoglobulin G; indirect competitive ELISA; Salmonella typhimurium