US EPA

1981120 

Journal Article 

Interleukin-8 and monocyte chemotactic protein-1 production by a human glioblastoma cell line, T98G in coculture with monocytes: involvement of monocyte-derived interleukin-1alpha 

Kasahara, T; Oda, T; Hatake, K; Akiyama, M; Mukaida, N; Matsushima, K 

1998 

Yes 

European Cytokine Network
ISSN: 1148-5493
EISSN: 1952-4005 

9 

1 

47-55 

English 

9613677 

WOS:000073621100004 

http:///www.john-libbey-eurotext.fr
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We have previously demonstrated that a human glioblastoma
cell line, T98G cells, produced high levels of interleukin-8 (IL-8) and monocyte chemotactic
protein-1 (MCP-1) when stimulated with IL-1 or tumor necrosis factor-alpha (TNF-alpha). In this
study, we found that T98G cells are capable of producing large amounts of IL-8 and MCP-1 when
cocultured with human peripheral blood monocytes or a monocytic cell line, U937 cells. Since it
is possible that both glioblastoma cells and monocytes are capable of producing chemokines, we
determined which type of cells actually produced IL-8 and MCP-1, by the fixation of one or the
other cell type with 3% paraformaldehyde (PA). This procedure revealed that T98G cells were the
main source and that PA-treated monocytes effectively stimulated IL-8 and MCP-1 production by
T98G cells. Both IL-8 and MCP-1 gene expression and protein production by T98G cells were
confirmed by northern blot as well as immunohistochemical staining methods. To analyze the
molecules on human monocytes responsible for inducing IL-8 and MCP-1 by T98G cells, several
antibodies (Abs) as well as IL-1 receptor antagonist (IL-1Ra) were tested, Anti-IL-1 alpha Ab and
IL-1Ra almost completely abolished the IL-8/MCP-1-inducing capacity of the PA-fixed monocytes,
while no inhibition was obtained with anti-IL-1 beta, anti-TNF-alpha or Abs against CD11b/18, L-
selectin or ICAM-1, indicating that membrane-associated IL-1 alpha is involved in the IL-8/MCP-1
induction, while secreted IL-1 alpha plays a major role in this cell-to-cell, i.e., juxtacrine
interaction in unfixed conditions. 

IL-8; MCP-1; glioblastoma; IL-1 alpha; IL-1 receptor antagonist