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20473 
Journal Article 
Thyroidal iodide transport II Comparison with non-thyroid iodide-concentrating tissues 
Wolff, J; Maurey, JR 
1961 
1 
Biochimica et Biophysica Acta
ISSN: 0006-3002
EISSN: 1878-2434 
47 
467-474 
English 
13786342 
#1. The iodide-concentrating mechanisms of normal sheep thyroid, mouse submaxillary, rat-mammary and rat-thyroid tumor tissue slices have been compared. 2. The tissue/medium ratio of 131I is greater for thyroid tissue than for the other tissues. 3. The time to attain equilibrium is 80-120 min in thyroid tissues and 30-40 min in the other three tissues. 4. The tissue/medium ratio of 131I of all four tissues is reduced to half the control value at 1 - 3 x 10-5 M iodide and the association constants vary from 2.7 - 3.3 x 10+4 M. The differences in tissue medium ratio of 131I appear to be a function of the capacity if the system rather than the affinity for iodide ions. 5. Iodide accumulation in all four tissues cab be shown to be a function of the external K+ concentration with half maximal stimulation of the tissue/medium ratio of 131I attained at from 0.9 - 2.9 x 10-3 M of added K+. 6. The cardiac glycosides, ouabain and scilliroside, depress the tissue/medium ration of 131I in all four tissues. Scilliroside is a more potent inhibitor than ouabain by an order of magnitude. This depression can be partially reversed by the addition of 30 mequiv./1 of K+, Rb+ or Cs+ ions.