US EPA

2064567 

Journal Article 

Mechanism of antiperspirant action by aluminum salts. Part 2. Histological observations of human eccrine sweat glands inhibited by aluminum chlorohydrate 

Quatrale, RP; Coble, DW; Stoner, KL; Felger, CB 

1981 

0 

Journal of the Society of Cosmetic Chemists
ISSN: 0037-9832 

IPA/81/455026 

Soc 

REF 7 

107-136 

eng 

IPA COPYRIGHT: ASHP Human forearm biopsy tissues containing eccrine sweat glands which were inhibited from firing by the occlusive application of aluminum chlorohydrate (aluminum chlorhydrate; I) were examined histologically by transmission electron microscopy to determine the presence and site of action of I within the sweat gland as a prerequisite to understanding its mechanism of action. The microscopy studies of the I treated glands revealed that the lumen of the duct at the level of the stratum corneum and stratum granulosum was completely filled with an electron dense amorphous material believed to consist of I or one of its reaction products and thought to be responsible for sweat gland inhibition. The untreated sweat glands were always patent and devoid of comparable material. Fluorescence microscopy studies of morin dye stained biopsy tissue revealed substantial fluorescence, indicating the presence of aluminum within the sweat gland duct at the level of the stratum corneum, and occasionally, at the stratum granulosum. Anatomically, the aluminum present within the sweat gland, as demonstrated with morin dye, was at the same site as the electron dense material demonstrated with transmission electron microscopy studies. Fluorescence was never observed in untreated sweat glands.