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2067173 
Journal Article 
Determination of serum and urinary aluminum by HPLC with fluorometric detection of Al-lumogallion complex 
Lee B-L; Chua L-H; Ong H-Y; Yang, HG; Wu, J; Ong C-N 
1996 
Yes 
Clinical Chemistry
ISSN: 0009-9147
EISSN: 1530-8561 
BIOSIS/96/34689 
42 
9 
1405-1411 
eng 
BIOSIS COPYRIGHT: BIOL ABS. We describe a simple and sensitive HPLC method for quantifying aluminum (Al) in biological fluids by measuring the fluorescence of the Al-lumogallion complex (excitation wavelength 500 nm, emission wavelength 575 run). Serum samples are deproteinized with 0.83 mol perchloric acid and centrifuged; the supernates are mixed with lumogallion reagent. Urine samples are pretreated with sodium hydroxide (2 mol/L) and methanol, kept for 1 h at -20? C, and then centrifuged; the precipitate is resuspended in perchloric acid and mixed with lumogallion reagent, as for serum. The maximal fluorescence complex is formed after 1 h at pH 5 | 0.5. The HPLC mobile phase consists of (per liter) 100 mL of 0.2 mol/L potassium hydrogen phthalate, 220 mL of acetonitrile, and distilled deionized water. The flow rate is 1 mL/min, and the injection volume is 5 muL. The major aluminum species is eluted at 3.5 min, the lowest detection limit being 0.45 pg. We validated the method with samples coll