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2071836 
Technical Report 
Interference of phytate with extraction of protein from brown rice using 5 M acetic acid 
Juliano, BO; Hussain, A; Resurreccion, AP; Bushuk, W 
1991 
BIOSIS/92/21251 
68 
3 
317-318 
eng 
BIOSIS COPYRIGHT: BIOL ABS. Patterns of 5 M acetic acid extracts of milled and brown rices, obtained by aluminum-lactate (AL) Polyacrylamide gel electrophoresis (PAGE) and reversed-phase high-performance liquid chromatography, showed the presence of major glutelin proteins in milled rice that were absent in brown rice. Phytate in the bran fraction was shown to selectively bind glutelin in 5 M acetic acid, since adding 1% sodium phytate to milled rice before extraction by acetic resulted in loss of some of the PAGE brands. Extraction of brown rice by 0.1 M sodium acetate buffer pH 5 to remove unbound phytate before extraction of protein by acetic acid resulted in an AL-PAGE pattern similar to that of milled rice.