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2073638 
Journal Article 
Thin-layer chromatographic and spectroscopic characterization of some diterpenes of the grayanotoxin type 
Kinghorn, AD; Jawad, FH; Doorenbos, NJ 
1978 
1 
Journal of Chromatography
ISSN: 0021-9673 
HEEP/78/09489 
147 1978 299 
299-308 
HEEP COPYRIGHT: BIOL ABS. In a study of toxic polyhydroxylated andromedane diterpenoids, several novel derivatives of grayanotoxin III (Rhododendron maximum) are described. Their spectral and physical characteristics are compared to ericaceous natural products. Separations on aluminium oxide and silica gel indicated that the absorption affinity of hydroxy groups on the perhydroazulene carbon skeleton was in the order 14beta-OH 3beta-OH 6beta-OH 2alpha-OH. Partition chromatography using ethylene glycol as stationary phase exhibited migration largely in accordance with the Reversed Traube's rule. This chromatographic-spectroscopic combination procedure is suitable for the micro-screening of these toxins.