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2079495 
Journal Article 
Orthophosphate and Phosphocreatine Content of the Myocardium 
Wollenberger, A; Krause, EG; Wahler, BE 
1958 
12 
1 
294 
The purpose of the experimental study was to ascertain the concentration of orthophosphate and phosphocreatine (67072) in the myocardium while avoiding the liberation of labilely bound orthophosphate in the sampling and processing of the tissue and during the process of analysis. A piece of the left cardiac apex of an adult dog and both heart chambers of an adult guinea pig were frozen in situ under pentobarbital (76744) anesthesia and artificial respiration by the rapid pressing together of 2 aluminum (7429905) blocks chilled to -180 degrees and forming the jaws of a pair of tongs, to yield sections 1 to 2 mm thick. (It is possible this way to obtain a considerably more rapid cooling than by immersion of uncompressed tissue in liquids that have been chilled to the same temperature but that possess a lower thermal conductivity than aluminum). The frozen tissue was ground to a very fine powder which revealed only cell fragments and analyses for orthophosphate and phosphocreatine undertaken. The analyses provided the lowest values found for cardiac orthophosphate from warm-blooded animals and the highest values for phosphocreatine. Simultaneous determinations of the total creatine (57001) content showed that in the tong-chilled myocardium of the guinea pig 50 percent, and in that of the dog more than 60 percent, of the creatine was present in phosphorylated form. 
DCN-177792; Cardiology; Enzymes; Enzymology; Histology; Cytology; Barbiturates; Cryogenics; Medications; 67-07-2; 76-74-4; 7429-90-5; 57-00-1