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2112262 
Journal Article 
The occurrence of tumours in F1, F2, and F3 descendants of pregnant mice injected with 7,12-dimethylbenz(a)anthracene 
Tomatis, L; Goodall, CM 
1969 
Yes 
International Journal of Cancer
ISSN: 0020-7136
EISSN: 1097-0215 
4 
2 
219-225 
English 
5347285 
Pregnant MA mice were injected intraperitoneally with 400 micrograms of 7,12‐Dimethylbenz(a)anthracene in olive oil during the last stages of pregnancy. The genetic design of the experiment included the follow‐up of the descendants for three consecutive generations and the cross‐mating between the F1 descendants of treated mothers with descendants of untreated controls. An increased incidence of tumours was observed in the female F1 and F2 descendants and the female F2f descendants (cross‐mating of F1 female descendants from a treated mother and male descendants from an untreated control). The tumours were observed in animals dying at a late age which approximately corresponded to the age when spontaneous tumours were observed in control animals. The most frequent types of tumours were the same as observed in the control animals, together with a handful of rarely observed tumours. In the F1 and F2 male descendants the tumour incidence was higher than in control animals but the difference was statistically not significant (binomial test). No significant increase in tumour incidence was observed in the F3 and F2m descendants (cross‐mating of F1 male descendants from treated mothers with female descendants from untreated controls). While the mice of F1 generation were directly exposed to small amounts of the chemical carcinogen via the mothers' milk and excreta, and possibly through the placenta, it seems unlikely that DMBA as such was transmitted to the F2 descendants. Copyright © 1969 Wiley‐Liss, Inc., A Wiley Company