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2115386 
Journal Article 
Separation of Lipid Classes by HPLC on a Cyanopropyl Column 
Olsson, P; Holmback, Jan; Herslof, B 
2012 
1 
Lipids
ISSN: 0024-4201
EISSN: 1558-9307 
47 
1 
93-99 
English 
22101922 
WOS:000299532400010 
A new method for the separation and identification of lipid classes by normal-phase HPLC on a cyanopropyl column is described. The use of a simple binary gradient, with toluene as a component, provided a rapid separation of non-polar as well as phospholipid classes. The inherent small differences in performances between possible non-polar eluent components of the gradient, such as hexane, heptane, and iso-octane, had a pronounced impact on retention times for individual phospholipid classes. Separation of molecular species within a lipid class could also be observed. 
Cyanopropyl column; Normal-phase HPLC; Lipid classes; Phospholipids; Toluene; Hexane; Heptane; Iso-octane