US EPA

2123264 

Journal Article 

Intestinal bacterial beta-glucuronidase activity of patients with colon cancer 

Kim, DH; Jin, YH 

2001 

Yes 

Archives of Pharmacal Research
ISSN: 0253-6269
EISSN: 1976-3786 

Pharmaceutical Society of Korea 

24 

6 

564-567 

English 

11794536 

10.1007/BF02975166 

WOS:000172928200017 

The fecal beta -glucuronidase activity of patients with Colon cancer and healthy controls were measured to determine the relationship between the fluctuation of intestinal bacterial beta -glucuronidase and colon cancer. The fecal beta -glucuronidase activity of patients with colon cancer was 1.7 times higher than that of the healthy controls. However, when these fecal specimens were sonicated, the enzyme activity of patients with colon cancer was 12.1 times higher than that of the healthy controls. The fecal beta -glucuronidase activity of human intestinal bacteria was drastically induced by its substrate or the bile secreted after a subcutaneous injection of 1,2-dimethyihydrazine (DMH) and benzo[a]pyrene into rats. DMH-and benzo[a]pyrene-treated biles induced beta -glucuronidase activity in the human intestinal microflora by approximately 1.5- and 2.3-fold, respectively. They also induced beta -glucuronidase in E coli HGU-3, which is a beta -glucuronidase-producing bacterium from the human intestine. D-saccharic acid 1,4-lactone similarly inhibited fecal beta -glucuronidase in several patients with colon cancer in addition to the healthy controls. This suggests that potent beta -glucuroniclase activity is a prime factor in the etiology of colon cancer. 

colon cancer; beta-glucuronidase; intestinal bacteria; 1,2-dimethylhydrazine