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2130635 
Journal Article 
Immunologic methods for the detection of benzo(a)pyrene metabolites in urine 
Gomes, M; Santella, RM 
1990 
Yes 
Chemical Research in Toxicology
ISSN: 0893-228X
EISSN: 1520-5010 
BIOSIS/90/25644 
3 
4 
307-310 
English 
2133077 
BIOSIS COPYRIGHT: BIOL ABS. Two monoclonal antibodies (10C10 and 4D5) have been developed from the spleen cells of Balb/c mice immunized with 6-aminobenzo(a)pyrene covalently coupled to bovine serum albumin. These antibodies have been used in an immunoassay for the detection of benzo(a)pyrene and its metabolites in mouse urine. The antibodies were characterized in terms of sensitivity and specificity by competitive enzyme-linked immunosorbent assay (ELISA). With both antibodies, 50% inhibition of antibody binding is at 4 pmol of BP. The antibodies also cross-react with a number of BP metabolites as well as with several other polycyclic aromatic hydrocarbons (PAHs) including pyrene, 1-aminopyrene, and 7,12-dimethylbenz(a)anthracene but with different sensitivities. These results suggest that this assay will detect multiple PAH metabolites in urine. To test the assay on biological samples, mice were treated with (3H)BP, and urine was collected and digested with beta-glucuronidase and aryl sulfatase.