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2130790 
Journal Article 
Phototoxicity testing in guinea-pigs 
Lovell, WW; Sanders, DJ 
1992 
Yes 
Food and Chemical Toxicology
ISSN: 0278-6915
EISSN: 1873-6351 
PERGAMON-ELSEVIER SCIENCE LTD 
OXFORD 
BIOSIS/92/15966 
30 
2 
155-160 
English 
1555796 
WOS:A1992HM53800009 
BIOSIS COPYRIGHT: BIOL ABS. The photoirritant potential of topically applied chemicals was studied using guinea-pigs. Solutions of test chemicals were applied to the skin, and after 30 min the animals were irradiated with near-ultraviolet radiation. Skin reactions were assessed subjectively between 3 and 72 hr after the start of treatment. Acridine and anthracene caused immediate photoirritation, whereas reaction to 8-methoxy-psoralen (8-MOP) and delayed; acridine was weakly active compared with the strong photoirritancy o anthracene and 8-MOP. Ethanol and a mixture of dimethylacetamide-acetone-ethanol (DEA) were satisfactory solvents, and a time interval of 15 to 30 min between application and irradiation was optimal. It is concluded that animal tests should not be recommended if ingredients have negligible absorption of sunlight. The safety hazard of ingredients absorbing near-ultraviolet and visible radiation may be assessed by laboratory animal procedures to satisfy governmental regulations. Th