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2143984 
Journal Article 
Effect of insulin on glucose transport in DMBA-induced mammary tumors 
Harmon, JT; Hilf, R 
1976 
Yes 
European Journal of Cancer
ISSN: 0959-8049
EISSN: 1879-0852 
HEEP/77/06975 
12 
11 
933-934 
English 
HEEP COPYRIGHT: BIOL ABS. Total glucose transport was examined in growing, static and regressing mammary tumors in the same diabetic rat. This animal, at the time of sacrifice, had blood glucose levels of 400 mg/100 ml and 10-11 M serum insulin levels as determined by radioimmunoassay. Insulin (10-10 M) stimulated or inhibited glucose transport in regressing or growing tumors, respectively. Insulin, under these conditions, had no effect on glucose transport in static tumors. While insulin stimulated glucose transport in normal cells, it consistently decreased glucose transport in dissociated R323OAC mammary tumor cells. Since this tumor is insulin independent, a DMBA (7,12-dimethylbenz(a)anthracene) induced tumor continuing to grow in a diabetic host might show a glucose transport response to insulin similar to the R323OAC tumor, which was the case with the present tumor. The regressing DMBA induced tumor showed stimulation of glucose transport in response to insulin in vitro. The lack of growth and glucose transport responses in the static tumor could be attributed to a heterogeneous cell population. Since all tumors were obtained from the same animal, it can be assumed that the divergence of the insulin response was not due to other hormonal influences, but rather to differences in tumor properties.