US EPA

2147649 

Journal Article 

Abstract 

Tissue binding of [9-14C] 2-acetylaminofluorene in mice during oral administration 

Jackson, CD; Weis, C; Shellenberger, TE 

1974 

1 

Toxicology and Applied Pharmacology
ISSN: 0041-008X
EISSN: 1096-0333 

29 

1 

107 

English 

WOS:A1974T653800088 

is part of a larger document 3378179 Abstracts of papers for the Thirteenth Annual Meeting of the Society of Toxicology, Washington, D.C. March 10–14, 1974

This study was made to determine the extent of tissue binding of 2-acetylaminofluorene (AAF) during oral administration of the carcinogen at different dose levels. Weanling, female BALB/cJ and C57BL/6J mice were maintained on diets containing 5 or 500 ppm [9-14C] AAF for 8 weeks. The amount of 14C bound to liver and bladder tissue, as well as blood concentrations, were determined at various intervals. Results obtained with the two dose levels were essentially parallel with amounts of 14C being approximately 100-fold higher in mice on the 500 ppm diet. Blood values of 14C in both strains of mice increased for 2 weeks and then remained constant with the concentrations in BALB/cJ mice being twice that of C57BL/6J mice on the same diet. Amounts of bound 14C in liver tissue also reached a plateau at 2 weeks and were approximately 40 % higher in C57BL/6J mice. In contrast, the amount of 14C bound to bladder tissue continued to increase over the entire 8-week period. In a second experiment, weanling, female BALB/cJ and C57BL/6J mice were maintained on diets containing AAF at various levels over the range of 0.5-500 ppm and sacrificed at the end of 2 weeks. Blood and tissue concentrations were determined as above. The results, plotted as log 14C bound vs log concentration of AAF in the diet, indicate that the amount of AAF bound to both liver and bladder tissue is linear over the dose range studied. 

Thirteenth Annual Meeting of the Society of Toxicology 

Washington, D.C. 

March 10–14, 1974