US EPA

2184842 

Journal Article 

An "in culture" bioassay to assess the estrogenicity of xenobiotics (E-SCREEN) 

Soto, AM; Lin, TM; Justicia, H; Silvia, RM; Sonnenschein, C 

1998 

Yes 

Journal of Clean Technology, Environmental Toxicology, and Occupational Medicine
ISSN: 1092-5732 

BIOSIS/98/25943 

7 

3 

331-343 

English 

WOS:000074688500007 

BIOSIS COPYRIGHT: BIOL ABS. An "in culture" bioassay was developed to assess the estrogenicity of xenobiotics using human breast estrogen-sensitive MCF7 cells. The assay compares the cell yield achieved after 6 days of culture in medium supplemented with 10% charcoal-dextran stripped human serum in the presence or absence of estradiol (10-13 to 10-9 M), and with diverse concentrations of xenobioticity suspected of being estrogenic. Among the chemicals tested phytohormones), the bioassay confirmed the estrogenicity of compound previously shown to be estrogenic in rodent models; no false positives were found. In addition, a case of estrogen contamination due to the shedding of estrogenic xenobiotics by polystyrene labware was solved using this assay. This bioassay has the following advantages over those currently used: (1) it is easy to perform; it requires a standard cell culture facility and a human estrogen-sensitive cell line; (2) it is relevant to humans, since it relies on the responses of a hu 

xenobiotic; in vitro; cell proliferation; estrogens; bioassay